Fig. 4.

Co-visualization of the Spitzenkörper and polarity markers in hyphae of Z. tritici. Hyphae of strains IPO323_CZtGFPMlc1, IPO323_CZtGFPSec4, IPO323_HeGFPRab11, and IPO323_CZtGFPExo70 were stained with FM4-64 (indicated in red), which is labeling the Spitzenkörper in other filamentous ascomycetes (Fischer-Parton et al., 2000). The myosin light chain Mlc1 (Mlc1, green), the small GTPases Sec4 (Sec4, green) and Rab11 (Rab11, green) are co-localizing with the FM4-64 signal at the tip of the hyphal cell. This localization confirms results in other ascomycete fungi (Sanchez-Leon et al., 2015; Pantazopoulou et al. 2014; Giraldo et al., 2013; Jones and Sudbery, 2010). The exocyst protein Exo70 does only partially co-localize with the Spitzenkörper (see merged image, lower right, Exo70 in green, FM4-64 in red). This is in agreement with a role of the exocyst in tethering secretory vesicles to the plasma membrane and confirms localization data in N. crassa and Candida albicans (Riquelme et al., 2014; Jones and Sudbery, 2010). Note that the cell edge is provided in blue. The bar represents 5 micrometers.