Fig. 2.

Absence of retinal projections in SCN and dLGN of math5 ^−/−. Anterograde labeling of retinal projections with CTB in the SCN and dLGN of WT (a, d) and math5 ^−/− (b, e) adult mice. Retinal projections are visualized by injecting CTB conjugated to Alexa 488 (green; contralateral projections) in one eye and Alexa 594 (red; ipsilateral projections) in the other eye. Shown are coronal sections depicting SCN and dLGN for WT (a, d) and math5 ^−/− (b, e). Inverted grey scale images in B and E depict the complete absence of CTB labeling in math5 ^−/−. (c, f) Corresponding DAPI images for sections (b) and (e). Dashed lines outline the boundaries of dLGN and SCN, and scale bar = 200 μm