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. 2015 Apr 7;16(1):269. doi: 10.1186/s12864-015-1375-x

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© Feng et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression levels of candidate CSPs in male and female S. litura antennae measured in single-end RNA-Seq (A) and RT-qPCR (B). In single-end RNA-seq, expression was calculated with log scale of RPKM value. The significant difference between female and male was justified by method of Audic and Claverie (1997) and indicated by symbol “*” (FDR < 0.01 and P < 0.05). In RT-qPCR, gene expression was calculated by the 2^-∆∆Cq algorithm with male as control, GAPDH and UCCR as reference genes. Female gene expression is presented normalized to male antennal expression arbitrarily defined as 1. Error bars signify SD. Significance of difference between male and female responses indicated by * P < 0.05, “#” means the significant difference between GAPDH and UCCR (P < 0.05), Students t test.