Figure 3.

Paclitaxel exposure upregulates the expression of TXNDC17 and autophagic proteins. A2780 (A), 3AO (B), and SKOV3 (C) cells were plated in 6-well plates overnight and reached 60% to 70% confluence when paclitaxel was added in the complete medium at final concentration 2, 5, and 10 nM. Total cell lysates were extracted after 3, 6, 12, 24, and 48 h of paclitaxel treatment. Protein levels of LC3B, ATG5, BECN1, and TXNDC17 were detected by western blotting. Cells without paclitaxel treatment were assigned as blank control (Blank). GAPDH was analyzed as the loading control. Data are representative of 3 independent experiments.