Figure 1.

PHF23 genetic information and expression profile. (A) Schematic of the gene and mRNA structure of PHF23. The PHF23 gene is located on chromosome 17, has 5 exons and encodes a protein with 403 amino acid residues. (B) Schematic representation of PHF23 and LRSAM1 constructs used in this study. (C) PHF23 mRNA expression was analyzed by RT-PCR in human normal tissues and cell lines (D). GAPDH expression was amplified as an internal control. (E) Protein expression of PHF23 in mammalian cell lines was detected by a rabbit anti-PHF23 antibody using western blot. ACTB was used as the loading control.