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. 2015 Jan 28;10(12):2158–2170. doi: 10.4161/auto.36439

Figure 5.

Figure 5.

PHF23PHDΔ increases cell autophagy. (A) Representative confocal microscopy images of endogenous LC3B distribution in U2OS cells transfected for 24 h with the indicated plasmids and then treated with or without CQ (25 μM) for the last 4 h. (B) Quantification of endogenous LC3B dots in U2OS cells treated as in (A). Data are means ± SD of at least 100 cells scored (*P < 0.05). (C) Western blot analysis of endogenous LC3B-II levels in U2OS cells transfected with the indicated plasmids for 24 h and then treated with or without CQ (25 μM) for the last 4 h. (D) Quantification of amounts of LC3B-II relative to ACTB in cells treated as in (C). The average value in the control vector-transfected cells without CQ treatment was normalized as 1. Data are means ± SD of results from 3 experiments (*P < 0.05, **P < 0.01). (E) Western blot analysis of endogenous SQSTM1 levels in U2OS cells transfected with vector or PHF23PHDΔ for 24 h. (F) Quantification of SQSTM1 levels relative to ACTB in cells treated as in (E). The average value in control vector-transfected cells was normalized as 1. Data are means ± SD of results from 3 experiments (*P < 0.05).