Figure 2.

ARG2 enhances TP53, and MTORC2-AKT-MTORC1-RPS6KB1 and impairs PRKAA signaling independently of its enzymatic activity. Young HUVECs were transduced as in Fig.1A. Sixty-4 h post transduction, cell lysates were prepared and subjected to (A) immunoblotting analysis of RICTOR, PRKAA-T172, and PRKAA, AKT-S473, and AKT, TP53-S15 and TP53, RPS6-S235/236 and RPS6, and tubulin. ((B)to F) Quantification of the signals is also shown (n = 4 as indicated in the figure). *P < 0.05, **P < 0.01, vs. V5-control group within the corresponding experimental condition. #P < 0.05, ##P < 0.01 vs. lane 1. †P < 0.05 vs. lane 7. To be clearer for readers, the 4 different experimental conditions were separated by dotted lines on the protein gel blot, but all conditions were run on the same gel.