Figure 4.

Overexpression of PRKAA1 in young HUVECs ameliorates ARG2-induced autophagy suppression. Young HUVECs were first transduced either with rAd/CMV as control or rAd/CMV-PRKAA1. Twenty-4 h after the 1st transduction, the cells were then transduced either with rAd/CMV as control (Con), rAd/CMV-Arg2 or rAd/CMV-H160F. Cell lysates were prepared 64 h (serum-free during the last 16 h) post the 2nd transduction for (A) immunoblotting analyses of PRKAA-T172 and PRKAA, LC3-I/-II, ATG12–ATG5, SQSTM1, and ARG2. Tubulin served as a loading control. (B) Quantification of the signals is shown in the bar graphs below (n = 4). **P < 0.01 vs. control; #P < 0.05, ##P < 0.01 vs. Arg2-cDNA + rAd/CMV.