Figure 3.

Comparison of in vitro transduction efficiency of recombinant adeno-associated virus (rAAV) recovered from CP, SN, or TC processes. (a) Light microscopy pictures of mSEAP-positive HEK293T cells 48 hours post-transduction. Compared to non transduced cells (top left), rAAV originating from TC process (bottom right) are able to efficiently transduce permissive cells and express the reporter gene as well as viral vectors recovered from the classical CP or SN processes (top right and bottom left respectively). Black points correspond to mSEAP activity revelation into transduced cells (see Materials and Methods for staining protocol). Scale bar = 40 µm. (b) Quantification of mSEAP activity in supernatant harvested from wells of transduced HEK293T cells. Despite the relative 2.5-fold increase of mSEAP concentration between TC and SN rAAV transduction results, this difference is still significant (with P < 0.01). Refer to Supplementary Table S2 for more details.