Figure 3.

Visualization of glycosome turnover by transmission electron microscopy. (A) Ultrathin sections of L. major promastigotes in stationary phase (left panel) or from log phase and starved in PBS (middle and right panels). Scale bars = 0.2 μm. Arrowheads mark double-membrane vesicle-forming autophagosomes; asterisks mark cytosol within vesicle; g, glycosome; m, mitochondrion. (B) L. major WT promastigotes were starved for 4 h in PBS and L. major Δatg5 were grown in normal medium prior to preparation for TEM. Arrows in left panels show the structures enlarged in the adjacent panels. In enlarged images: arrowheads label autophagosomes; g, glycosome.