Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 28;10(12):2143–2157. doi: 10.4161/auto.36438

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Author(s). Published with license by Taylor & Francis

© Benjamin Cull, Joseane Lima Prado Godinho, Juliany Cola Fernandes Rodrigues, Benjamin Frank, Uta Schurigt, Roderick AM Williams, Graham H Coombs, and Jeremy C Mottram

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 6. — Characterization of vesicle size in L. major during stationary phase. (A) L. major expressing GFP-ATG8 were incubated with 40 μM FM 4–64 for 1 h to stain the lysosomal compartment, before imaging. Left: Average vesicle size. Dots represent the mean and the error bars standard deviation. Right: Frequency distribution of vesicle sizes. (B) L. major coexpressing GFP-ATG8 and proCPB-RFP. Left: Average vesicle size. Dots represent the mean and the error bars standard deviation. Right: Numbers of tubular compartments labeled with GFP-ATG8, proCPB-RFP or both. Bottom: Frequency distribution of vesicle sizes. Vesicle sizes were measured using SoftWoRx Explorer Image analysis software. Data were analyzed using the Student t test (for 2 samples; A) or one-way ANOVA with a Tukey-Kramer multiple comparison test (for 3 samples; B). Frequency distribution data were binned by 0.065 μm intervals, which correspond to the limit of measurement accuracy based on the microscopy image pixels. *** denotes P < 0.001.