Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 28;10(12):2143–2157. doi: 10.4161/auto.36438

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Author(s). Published with license by Taylor & Francis

© Benjamin Cull, Joseane Lima Prado Godinho, Juliany Cola Fernandes Rodrigues, Benjamin Frank, Uta Schurigt, Roderick AM Williams, Graham H Coombs, and Jeremy C Mottram

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 7. — Analysis of autophagy and the mitochondrion during normal growth and starvation. (A) Stationary phase L. major promastigotes coexpressing RFP-ATG8 and ROM-GFP. (B) The occurrence and colocalization of mC-ATG5 or RFP-ATG8 puncta and MUP-GFP coexpressed in log phase WT L. major promastigotes after 4 h in PBS at 25°C. RFP-ATG8 or mC-ATG5 puncta colocalizing with MUP-GFP are arrowed. Images show a single slice from a 3-μm Z stack. Scale bar = 5 μm. (C) TEM. Ultrathin sections of L. major promastigotes after starvation in PBS. Arrowheads mark double-membrane vesicles formed around pieces of mitochondrion. Arrows mark ER profiles which may represent forming autophagic membranes. K, kinetoplast; LB, lipid body; m, mitochondrion; N, nucleus. Scale bars = 0.5 μm.