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. 2015 Feb 20;11(2):355–372. doi: 10.4161/15548627.2014.994368

Figure 1.

Figure 1.

Inhibiting the Hh pathway decreased cell viability of BCR-ABL+ CML cells. (A and B) K562 cells were treated with 10, 20, and 40 μM of vismodegib for 24 h, gene expression of PTCH (A) and GLI1 (B) were detected by quantitative RT-PCR. Data shown are mean ± SD of triplicates of one typical experiment. Similar results were obtained from 3 independent experiments * versus Control, P < 0.05, ** vs. to Control, P < 0.01. (C) K562 cells were treated with 10, 20, and 40 μM of vismodegib for 48 h, protein levels of GLI1, CCND1, MYC, p-GSK3B, GSK3B, CTNNB1, and ACTB were determined by western blot assay. Densitometric values were quantified using the ImageJ software and normalized to control. The values of control were set to 1. The data represents the mean of 3 independent experiments. (D) K562, BaF3-BCR-ABL, BaF3-BCR-ABLT315I, and BaF3-BCR-ABLY253F cells were treated with 2.5, 5, 10, 20, and 40 μM of vismodegib for 48 h, cell viability was determined by the CCK-8 assay.