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. 2015 Feb 20;11(2):355–372. doi: 10.4161/15548627.2014.994368

Figure 2.

Figure 2.

Inhibiting the Hh pathway induced autophagy in CML cells. (A) K562, BaF3-BCR-ABL, BaF3-BCR-ABLT315I, and BaF3-BCR-ABLY253F cells were treated with 20 μM of vismodegib for 48 h, autophagosomes were observed using transmission electron microscopy. Arrows represent autophagosomes. (B and C) K562, BaF3-BCR-ABL, and BaF3-BCR-ABLT315I cells were transiently transfected with an eGFPMAP1LC3B plasmid for 24 h, followed by treatment of 20 μM of vismodegib for 48 h. Cells were observed under confocal microscopy and typical images are presented (B). Green fluorescence spots represent MAP1LC3B-II spots. MAP1LC3B-II spots were counted using ImageJ software (C). ** P < 0.01. n = 20.