Figure 3.
Inhibiting the Hh pathway induced autophagic flux in CML cells. (A) K562 and BaF3-BCR-ABL cells were treated with 20 μM of vismodegib for 6 h, 12 h, 24 h, or 48 h, protein levels of SQSTM1, MAP1LC3B, and ACTB were measured by western blot assays. (B) BaF3-BCR-ABLT315I and BaF3-BCR-ABLY253F cells were treated with 20 μM of vismodegib for 6 h, 12 h, 24 h, or 48 h, protein levels of SQSTM1, MAP1LC3B, and ACTB were measured by western blot assays. (C) K562, BaF3-BCR-ABL, BaF3-BCR-ABLT315I, and BaF3-BCR-ABLY253F cells were cotreated with vismodegib (20 μM) and Bafi A1 (5 nM) or treated with these 2 agents alone for 48 h, protein levels of SQSTM1, MAP1LC3B, and ACTB were measured by western blot assays. (A–C) Densitometric values were quantified using the ImageJ software and normalized to control. The values of control were set to 1. The data are presented as means ± SD of 3 independent experiments.