Figure 7.

ATG5 and ATG7 silencing significantly enhanced cell death induced by Hh inhibition in CML cells. (A) K562 cells were transiently transfected with ATG5 or ATG7 siRNAs for 48 h, the protein levels of ATG5, ATG7, and ACTB were detected by western blot assays. (B) After K562 cells were transiently transfected with ATG5 or ATG7 siRNAs for 24 h, cells were treated with 20 μM of vismodegib for 12 h, 24 h, and 48 h, the protein levels of SQSTM1, MAP1LC3B, and ACTB were measured by western blot assay. (C) K562 cells were treated as described in (B), cell viability was determined by CCK-8 assay. (D) BaF3-BCR-ABL^T315I cells were treated as described in (A), the indicated protein levels were detected by western blot assay. (E) BaF3-BCR-ABL^T315I cells were treated as described in (B), the indicated protein levels were detected by western blot (E) and cell viability was measured by CCK-8 assay (F). Relative expression levels in (A, B, D and E), shown below the blot are representative of 2 independent experiments. Densitometric values were quantified using the ImageJ software and normalized to control. The values of control were set to 1. Data in (C and F) are means ± SD of 3 independent experiments performed in triplicate. **P < 0.01.