Figure 9.

Simultaneous inhibition of the Hh pathway and autophagy significantly induced apoptosis in CML cells. (A) K562, BaF3-BCR-ABL, and BaF3-BCR-ABL^T315I cells were cotreated with vismodegib (20 μM) and CQ (10 μM) for 12 h, 24 h, and 48 h. The expression levels of PARP, CASP9, CASP3 and ACTB were analyzed by western blot. (B) K562, BaF3-BCR-ABL, and BaF3-BCR-ABL^T315I cells were treated as described in (A), apoptosis was detected by ANXA5 and PI staining and flow cytometry. The percentages of ANXA5⁺ PtdIns⁻ cells (apoptosis) and ANXA5⁺ PI⁺ cells (necrosis) are presented in bar charts. Error bars represent SD from triplicate. A typical result from 3 independent experiments is presented. *P < 0.05; **P < 0.01; ***P < 0.001.