Figure 11.
Simultaneous inhibition of the Hh pathway and autophagy also inhibited the kinase activity of BCR-ABL oncoprotein in BCR-ABL+ CML cells. K562 and BaF3-BCR-ABLT315I cells were cotreated with vismodegib (20 μM) and CQ (10 μM) for 48 h. (A) The protein levels of p-BCR-ABL and p-STAT5 were analyzed by western blot. (B and C) The relative protein expression levels are shown in bar chart. Densitometric values were quantified using the ImageJ software and normalized to control. The values of control were set to 1. Data are means ± SD of 3 independent experiments.