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. 2014 Dec 18;10(11):2053–2074. doi: 10.4161/15548627.2014.973737

Figure 7.

Figure 7.

Effects of in vivo treatment on BECN1 and SQSTM1 protein levels in nasal brushing from F508del-CFTR homozygous patients. (A) Effects of treatment on BECN1 (left) and SQSTM1 (tight) protein levels in freshly isolated nasal epithelial cells of patients No. 5, 6 and 10 of Table 1. The values are expressed as percentage of non-CF healthy control (considered as 100% of value). Mean ± SD of triplicates of independent experiments per each patient's sample; ##p < 0.01 compared to non-CF healthy control, °P < 0.05 and °°P < 0.01 versus wk 0 (ANOVA). (B and C) Representative blot of 1 patient (No. 10 of Table 1) out of 3 patients analyzed. (B) Western blot analysis of cell lysates and immunoblot with anti-BECN-1 (Abcam); (C) Western blot analysis of insoluble and soluble protein fractions and immunoblot with anti-SQSTM1 (Sigma Aldrich). ACTB was used as negative marker of the insoluble protein fraction and as loading control of the experiment. Bottom, densitometric measurement of (B) BECN-1 and (C) SQSTM1 levels expressed as percentage of non-CF healthy control. Mean ± SD of triplicates of independent experiments; ##P < 0.01 compared to non-CF healthy control, °P < 0.05 and °°P < 0.01 versus wk 0 (ANOVA).