Figure 9.

Dual roles of AMPK in cytoprotective and cytostatic autophagy. (A) H460 cells were exposed to 6 Gy of radiation (IR) or EB 1089 (100 nM) + radiation and phosphorylation of AMPK (Thr172) was monitored by flow cytometry at 24 h post-radiation. (B) Western blotting was performed to confirm successful inhibition of phosphorylation of AMPK and its downstream target ACC (acetyl-CoA carboxylase) by using compound C (inhibitor of AMPK). (C/D) Effect of compound C on the temporal response to radiation (IR, 6 Gy) alone (C)or EB 1089 + radiation (D) was assessed by monitoring cell viability. n = 3, mean ± SE, *P < 0.05, #P < 0.001.