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. 2014 Dec 18;10(11):1921–1936. doi: 10.4161/15548627.2014.981790

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© 2014 The Author(s). Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — ER stress-induced cell death in apoptosome-compromised cells is accompanied by caspase activation. (A and B) Casp9^+/+ and casp9^−/− MEFs were treated for the indicated times with (A) 0.5 μM Tg or (B) 0.5 μg/ml of Tm and lysates immunoblotted for CASP9, CASP3, PARP, and actin. (C) Bax^+/+ Bak1^+/+ and bax^−/− bak1^−/− MEFs were treated with 1 μg/mL of Tm for indicated timepoints. Cell viability was analyzed by PI uptake. (D) Bax^+/+ Bak1^+/+ and bax^−/− bak1^−/− MEFs were treated with 1 μg/ml of Tm for the indicated times and lysates were immunoblotted for CASP9, CASP3, PARP and tubulin. (E) Bax^+/+ Bak1^+/+ and bax^−/− bak1^−/− MEFs were treated with 1 μg/ml of Tm for indicated times and CASP3-like activity was determined by DEVD-AMC hydrolysis. Results are representative of at least 3 independent experiments. Error bars represent the mean ± SD. (G) casp9^−/− MEFs were treated with 0.5 μM Tg with or without Boc-D-FMK for the indicated times and lysates immunoblotted for PARP and cleaved CASP3. (H and I) casp9^−/− MEFs were treated with (H) 0.5 μM Tg or (I) 0.5 μg/ml of Tm for the indicated times and lysates immunoblotted for cleaved CASP8.