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. 2014 Oct 30;10(11):1895–1905. doi: 10.4161/auto.32200

Figure 3.

Figure 3.

Vacuolin-1 inhibited general endosomal-lysosomal degradation in HeLa cells. (A) The processing of CTSL from the precursor form to its mature form in HeLa cells treated with or without vacuolin-1 (1 μM) or BAF (100 nM). (B) Vacuolin-1 (1 μM) inhibited EGF-induced EGFR degradation in HeLa cells. (C) Vacuolin-1 (1 μM) inhibited the fusion of EGFR endosomes with lysosomes in HeLa cells. Scale bar: 20 μM. The EGF-488/LAMP1 colocalization coefficiency is expressed as mean ± S.E., n = ∼40 cells of 2 independent experiments. (D) Vacuolin-1 (1 μM) inhibited the degradation of DQ-BSA-Green in HeLa cells. (E) Vacuolin-1 nonspecifically inhibited V-ATPase as assessed by the in vitro V-ATPase assay, whereas BAF markedly inhibited it. The activity of V-ATPase (1.05 ± 0.09 μmol/min/mg) at 0 μM of inhibitors is set to 100%. The data are expressed as mean ± S.D., n = 3. The * symbols indicate the results of the Student t test analysis, P < 0.05.