Figure 3.

Colocalization of the P140 peptide with endolysosomal markers and components. (A, C, E, G) MRL/lpr mice received Alexa Fluor 488 labeled-P140 peptide (100 μg/mouse) by the iv route. Splenocytes were collected 3 h later and stained for the early endosomal marker EEA1 (A), or 6 h later and stained for the late endosomal marker RAB9 (C), lysosomal component LAMP2 (E) or LAMP2A isoform (G). Representative results of 3 independent experiments are shown. (B, D, F, H) B cells from MRL/lpr mice were incubated for 15 min (B) or 2 h (D, F, H) in the presence of Alexa Fluor 488 labeled-P140 peptide and stained for EEA1 (B), RAB9 (D) LAMP2 (F) or LAMP2A isoform (H). Representative results of 2 to 4 independent experiments are shown (2 different experimenters).