Figure 4.

Effect of the P140 peptide on CMA activity on mouse fibroblasts. NIH3T3 cells stably expressing the photoactivable CMA reporter KFERQ-PA-mCherry1 were photoactivated and maintained in serum-free medium supplemented with or without (w/o) with the indicated peptides for 12 h. (A to D) Representative images, low magnification field and higher magnification single cell inserts of cells that were kept untreated (A), or treated with the P140 peptide (B), scrambled peptide ScP140 (C) or unphosphorylated peptide 131 to 151 used as control (D). Nuclei were highlighted by DAPI. (E) Quantification of the average number of fluorescent puncta per cell. Values are mean + standard error of the mean (SEM). N > 50 cells. P values are indicated (Student t test).