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. 2015 Jan 28;10(12):2333–2345. doi: 10.4161/15548627.2014.984275

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© 2014 The Author(s). Published with license by Taylor & Francis

© Sanda Mimouna, Marie Bazin, Baharia Mograbi, Arlette Darfeuille-Michaud, Patrick Brest, Paul Hofman, and Valérie Vouret-Craviari

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Pangenomic microarray profiling HIF1A-induced xenophagic genes. (A) A microarray analysis reveals genes from the HIF and from phagocytosis and lysosome GSEA projection as a transcriptional target of HIF1A. Control and HIF1A-silenced cells were infected with AIEC LF82 bacteria for a 4 h-period and total RNA was extracted. The samples were then cohybridized to a pangenomic microarray. VAV2, CTSV, ATP6V1H and ATP6V1E downregulated expressions were detected by microarray. Hybridizations were performed in duplicate. (B) Quantification of the selected gene mRNA levels was measured by RT-PCR in AIEC LF82-infected T84-ShCTR and ShHIF1A cells. The inhibition of mRNA levels was confirmed for all the selected genes. Results shown are representative of 2 separate experiments made in duplicate.