Figure 5.

HIF1A does not impair autophagy. (A) Nutrient stress-induced autophagy was characterized by immunoblot analysis with LC3-II antibody of cellular lysates from T84-ShCTR and T84-ShHIF1A incubated in HBSS for 0, 30 and 60 min, then lysed and subjected to sonication. The time course analysis indicates that the autophagic flux is functional in both cell lines. The data are representative of 3 independent experiments. (B) Mitophagy was assessed in T84-ShCTR and T84-ShHIF1A cells using a MitoTracker Red. Representative microscopy images show almost no mitochondria under hypoxia in control cells. By contrast, and as expected for a HIF1A-dependent response, mitochondria were stained by MitoTracker Red in HIF1A-silenced cells under normoxia and hypoxia.