Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 20;11(1):166–182. doi: 10.4161/15548627.2014.981915

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Majid Sakhi Jabir, Lee Hopkins, Neil D. Ritchie, Ihsan Ullah, Hannah K. Bayes, Dong Li, Panagiotis Tourlomousis, Alison Lupton, Daniel Puleston, Anna Katharina Simon, Clare Bryant, and Thomas J. Evans

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 6. — Mitochondrial DNA is present in NLRC4 immunoprecipitates and activates a reconstituted NLRC4 inflammasome. (A) BMDMs were grown in BrdU and infected as shown before lysates were immunoprecipitated with anti-NLRC4 or control rabbit serum as indicated. Bound material was slot-blotted to nitrocellulose and then blotted with anti-BrdU. The lower panel shows separate immunoblot of eluted material from NLRC4 immunoprecipitates blotted for NLRC4. (B) As in (A), but reprobed with antibody to 8OHdG. (C) As (A), but blot probed with generic anti-DNA antibody. (D) Immunoblot of immunoprecipitates as set out in (A) but in either control J774A.1 cells or in ρJ774A.1 cells lacking mitochondria. (E) HEK cells transfected with NLRC4 and NAIP were transfected with and without mitochondrial DNA and active CASP1 localized by immunofluorescent imaging using FLICA^Casp1. Panels show representative images of cells stained with FLICA^Casp1 (green) and nuclei stained with DAPI (blue) using the indicated transfection reagents. Arrows show spots of active CASP1 formation. Experiments were all repeated 2 or 3 times.