Figure 1.

Association of cilium length and autophagy from cell growth to differentiation. To determine the association of cilium length and autophagy, HK2 cells were cultured in DMEM-F12 medium containing 10% FBS at subconfluence (Sub-CF), confluence (CF), or for one or 3 d after reaching confluence (Post-CF d 1, 3). (A) Cells were assayed by immunofluorescence staining with the antibody to Ac-TUBA (red) and DAPI (blue) and cell lysates examined by immunoblot analysis of LC3B and ACTB. Scale bar: 5 5 μ. (B) Cilium length was averaged out of over 50 cilia in each group (Sub-CF, n=52; CF, =56; Post-CF d 1, =51; Post-CF d 3, =60). Cilium length: Post-CF d 3>Post-CF d 1>CF>Sub-CF, * P < 0.05. (C) Expression level of KIF3A and IFT88 from cell growth to differentiation. Please note, as previously reported,⁷ 2 forms of IFT88 were detected. (D) Positive correlation of cilium length with LC3B-II/-I or LC3B-II/ACTB ratio.