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. 2015 Jan 21;11(2):214–224. doi: 10.4161/15548627.2014.994400

Figure 1.

Figure 1.

Inhibition of HMGB1 expression and cytoplasmic translocation enhance TNFSF10-mediated cell death. (A) The indicated HMGB1 wild-type (control shRNA) and knockdown (HMGB1 shRNA) cells were treated with TNFSF10 (1 to 1000 ng/ml) for 24 h, and then cell viability was assayed with the CCK-8 kit (n = 3 , *, P < 0.05). Western blot analysis of the expression of HMGB1 is shown in the inserts. (B) PANC-1 and Jurkat cells were treated with TNFSF10 (100 ng/ml) for 24 h and then cytosolic (Cyt) and nuclear (Nuc) HMGB1 were assayed by western blot. Relative band intensities of cytosolic HMGB1 were quantified and normalized to actin expression (P < 0.05). AU, arbitrary unit. (C and D) PANC-1 cells were treated with TNFSF10 (100 ng/ml) in the absence or presence of indicated ethyl pyruvate (EP) for 24 h. Cytosolic (Cyt) HMGB1 (C) was assayed by western blot and cell viability (D) was assayed by CCK-8 kit (n = 3 , *, P < 0.05). AU, arbitrary unit.