Skip to main content
. 2014 Nov 14;11(1):46–59. doi: 10.4161/15548627.2014.984271

Figure 1.

Figure 1.

Metabolic effects of metformin and caloric restriction on ob/ob mice. The effects of metformin and CR on body weight (A), food intake (B), and blood glucose (C) in the 8-wk-old C57bl/6j control (○) and ob/ob mice were evaluated. The latter mice were classified into 3 groups: ad libitum feeding of chow diets (▴), 300 mg/kg intraperitoneal metformin (▵), and 3 g/d CR (Ø). During a 4-wk treatment course of either metformin or CR, body weight significantly decreased compared to ad libitum-fed ob/ob mice (A). Due to the protocol of this study, food intake was only reduced in calorie-restricted mice. Blood glucose levels were significantly decreased throughout the entire 4 wk in ob/ob mice treated either with metformin or CR, compared to ad libitum-fed ob/ob mice (C). At 3 wk, glucose tolerance tests were performed after an intraperitoneal injection of glucose (2 g/g body weight, D). Blood was collected from the tail vein, and glucose concentrations were measured at 0, 15, 30, 60, 90, and 120 min. CR significantly improved glucose tolerance throughout the IPGTTs, but metformin only significantly improved glucose tolerance at 60, 90, and 120 min. Values displayed are means ± SEM of 8 mice per group.***P < 0.001, CR vs. ad libitum-fed ob/ob mice;`P < 0.05,††P < 0.01, and†††P < 0.001, metformin vs. ad libitum-fed ob/ob mice.