Figure 1.

Pilocarpine induced in vivo salivation from mouse parotid (PG), submandibular (SMG), and sublingual glands (SLG). (A) Flow rate (µL/min) of pilocarpine (10 mg/kg)-stimulated, gland-specific saliva was collected for 30 min from PG (open squares), SMG (filled circles), and SLG (gray triangles). (B) Total amount of secreted saliva during 30 min of stimulation (µL/30 min). Secreted saliva was significantly greater in PG, followed by SMG and SLG. (C) Average gland weight (mg) was significantly greater in SMG, followed by PG and SLG. (D) Amount of saliva normalized by gland weight (µL/mg). Saliva amount per gland weight was significantly greater in PG than in SMG and SLG, while SMG and SLG were not different. One-way ANOVA followed by Bonferroni’s post hoc test was applied for the detection of statistically significant differences. *P < 0.05; n = 24 (males = 12, females = 12).