Figure 3.

Overexpression and knockdown of FoxO1 can affect the expression of p53-upregulated modulator of apoptosis (PUMA). A, Quantitative real-time polymerase chain reaction (RT-PCR) showed the messenger RNA (mRNA) transcription changes of FoxO1 in response to 100 μmol/L H₂O₂ treated for 24 hours in cultural follicular granulosa cell (GCs). B, Western blot of FoxO1 protein level in cultural follicular GCs after treatment with 200 μmol/L H₂O₂ for 36 hours. An internal control was served by α-Tubulin. C, Overexpression of FoxO1 and elevated PUMA protein levels were detected by Western blot. D, Western blot of FoxO1 and PUMA protein levels after treatment with H₂O₂ in the presence of scramble-shRNA or FOXO1-shRNA. An internal control was served by α-tubulin. * indicates P < .05; ** indicates P < .01.