Figure 4.

Oxidative stress-induced increase in p53-upregulated modulator of apoptosis (PUMA) expression can be regulated by FoxO1 activation. A, Representative illustrations for FoxO1 translocation are listed. FoxO1 was visualized by green staining. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue). Quantitative analysis for FoxO1 cellular localization was counted by randomly selecting 6 slides. Bar = 5 μm. B, Quantitative PCR of PUMA messenger RNA (mRNA) level after treatment with 100 μmol/L H₂O₂ in the presence or absence of 15 μmol/L SP600125. Different letters denote significant differences between each groups (P < .05). C, Western blot of PUMA protein level after treatment with 200 μmol/L H₂O₂ in the presence or absence of 15 μmol/L SP600125. * indicates P < .05; ** indicates P < .01.