Figure 2.
Sildenafil rescue of HTR cells through NO and cGMP signaling. HTR cells were treated with H/R and medium was supplemented as indicated with 10 μmol/L cGMP analogue, 350 ng/mL sildenafil with or without 10 μmol/L cGMP inhibitor, 10 μmol/L l-NAME, or 10 μmol/L d-NAME. Medium was also supplemented with 10 μmol/L SNAP with or without cGMP inhibitor. Cells were labeled for TUNEL and the TUNEL Index was determined. Error bars denote SEM *P < .05 compared to vehicle. HTR indicates human trophoblast cells; H/R, hypoxia/reoxygenation; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling; l-NAME, l-NG-Nitro-l-arginine Methyl Ester Hydrochloride SEM, standrd error of the mean; SNAP, S-Nitroso-N-acetylpenicillamine.