Table 1.
Primer Sequences for Genes Studied.
| Gene | NCBI Acc No | Primer Sequence (5′-3′) |
|---|---|---|
| NOS1 | XM_867630 (Bt) | Forward: AAACCACCAGCACCTACCAG |
| Reverse: TCTGAGGTTCCCTTTGTTGG | ||
| NOS2 | NM_001076799 (Bt) | Forward: AAGGCAGCCTGTGAGACATT |
| Reverse: CAGATTCTGCTGCGATTTGA | ||
| NOS3 | NM_181037 (Bt) | Forward: TCTTCCACCAGGAGATGGTC |
| Reverse: AGAGGCGTACAGGATGGTTG | ||
| NRF-1 | XM_004008056.1 (Oa) | Forward: AAACACAAACACAGGCCACA |
| Reverse: CACCGCCGAATAATTCACTT | ||
| NRF-2 | AY369137.1 (Oa) | Forward: GCAGCCGGTGTGAGTAGAGATG |
| Reverse: TGGTGTTGCAGCATGTATCGTC | ||
| mtTFA | GAAI01000507.1(Oa) | Forward: CAAATGATGGAAGTTGGACG |
| Reverse: AGCTTCCGGTATTGAGACC | ||
| COX-I | NC_001941.1 (Oa) | Forward: GAACTCTGCTCGGAGACGAC |
| Reverse: GGGGTGTCCAAAGAATCAGA | ||
| COX-II | NC_001941.1 (Oa) | Forward: TTCCAAGATGCAACATCACC |
| Reverse: GGGCAGAATGGTTCAGATTG | ||
| COX-IV | XM_004014950.1 (Oa) | Forward: AGGAGAAGATGCCTCCCTACA |
| Reverse: GGTTCATCTCTGCGAAGGTC | ||
| Ki67 | XM_004004769.1 (Oa) | Forward: TCAGTGAGCAGGAGGCAGTA |
| Reverse: GGAAATCCAGGTGACTTGCT | ||
| PCNA | XM_004014340.1 (Oa) | Forward: GTGTTTTGCCTCTCGCTCTC |
| Reverse: GAAGGGTTAGCTGCACCAAG | ||
| GR | NC_007305.5 (Bt) | Forward: GTGCAGAATCTCATAGGCTGCC |
| Reverse: TGTGTGGTACGCCTTCTGATT | ||
| Cyclophilin | BT020966 (Bt) | Forward: CCATCGTGTCATCAAGGACTTCAT |
| Reverse: CTTGCCATCCAGCCAGGAGGTCTT |
Abbreviations: NOS1, neuronal nitric oxide synthase; NOS2, inducible nitric oxide synthase; NOS3, endothelial nitric oxide synthase; NRF-1, nuclear respiratory factor 1; NRF-2, nuclear respiratory factor 2; mtTFA/TFAM, mitochondrial transcription factor A; COX-1, cytochrome c oxidase subunit I; COX-II, cytochrome c oxidase subunit II; COX-IV, cytochrome c oxidase subunit IV; Ki67, MKI67 (FHA domain) interacting nucleolar phophoprotein; PCNA, proliferating cell nuclear antigen; GR, glucocorticoid receptor; Bt, Bos taurus; Oa, Ovis aries.