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. 2014 Nov 14;11(1):88–99. doi: 10.4161/15548627.2014.984277

Figure 2.

Figure 2.

Chloroquine and monensin induce LC3 lipidation onto multiple lysosomal compartments dependent on V-ATPase activity. (A and B) Confocal images of GFP-LC3 and LAMP1 immunostaining of (A) lysosomes in MCF10A and (B) uncoated latex bead phagosomes in J774 macrophage following treatment with Baf (100 nM), CQ (100 mM) or Baf + CQ for 1 h. Arrow indicates GFP-LC3 lipidation onto a phagosome. Bar = 6 μm. (C and D) Images of GFP-LC3 and LAMP1 on entotic corpse vacuoles in MCF10A cells treated with Baf, CQ, Mon (100 μM) or Baf + CQ for 1 h. Arrows indicate GFP-LC3 lipidation onto vacuoles. Bar = 10 mm. (E) GFP-LC3 and ATP6V0D1 (V0D1) staining on entotic corpse vacuoles with our without CQ treatment. Bar = 10 μm. (F) GFP-LC3G120A and LAMP1 staining on entotic corpse vacuoles following CQ treatment. Bar = 10 μm. (G) GFP-LC3 and ATG5 immunostaining on entotic corpse vacuole (arrows) following CQ treatment. Bar = 10 μM. (H) (i) Electron microscopy of corpse containing cell-in-cell structure treated with CQ (100 μM), (ii) entotic corpse vacuole has a single membrane (arrows). See also Figure S3; Movie S1.