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. 2014 Nov 14;11(1):88–99. doi: 10.4161/15548627.2014.984277

Figure 6.

Figure 6.

LC3 recruitment during LAP and entosis is dependent on V-ATPase activity. (A) Quantification of entotic inner cell death over 20 h with or without Concanamycin A (ConA, 100 nM). Data are mean ± SEM of 3 separate experiments; P < 0.05 *. (B and C) Quantification of LC3 recruitment to entotic vacuole with or without ConA (100 μM) during (B) nonapoptotic or (C) apoptotic death of inner cells. Data are mean ± SEM of 3 separate experiments; P < 0.002 **; N/A, not applicable. (D and E) Quantification of GFP-LC3 recruitment to (D) zymosan phagosomes in RAW264.7 cells and (E) IgG-coated bead phagosomes in IFNG-treated RAW264.7 cells. Data are mean ± SEM of 3 separate experiments; P < 0.002 **. (F and G) Confocal time-lapse images of GFP-LC3 and 2xFYVE-mCherry in RAW264.7 cells during zymosan phagocytosis with or without Baf (100 μM). Arrows point to 2xFYVE recruitment and GFP-LC3 lipidation as indicated. Bar = 3 μm.