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Punctate GFP-LC3B in replicative senescent human fibroblasts. (A) Growth curves of primary WI-38 and WI-38-TERT fibroblasts in extended culture. Error bars represent standard deviation among 3 wells grown in parallel. (B) Representative images of GFP-LC3B puncta in WI-38 or WI-38-TERT cells at passage 1 and 19 after initiation of culture. A 40X objective was used for imaging. * and ** indicate significance at P < 0.05 and P < 0.01 respectively.
