Figure 4.
Autophagy inhibition does not affect the senescence transition following MT-HsTER treatment of BJ fibroblasts. (A) LC3B immunoblotting in BJ-TERT fibroblasts transduced with a control, ATG5, or ATG7 shRNA 7 d after MT-HsTER addition. Relative amounts of LC3-II are indicated below the blots. (B) SA-β-gal staining 7 d after MT-HsTER addition. SA-β-gal staining showed a significant increase between vector and MT-HsTER cells (P < 0.05) but no significant differences were observed between the MT-HsTER-treated samples. Error bars represent standard error. (C) Crystal violet-stained colonies showing colony forming efficiency upon plating 3 × 103 cells at day 8 after MT-HsTER addition. Data is representative of at least 3 independent experiments.