Figure 8.

There was increased accumulation of lipid-filled vacuoles within the RPE of the Rb1cc1-CKO mice as compared to littermate controls. (A–C) Sections from eyes of 8-mo-old mice stained with Oil Red O show the disruption of the normal RPE architecture the presence of large, lipid-filled structures (yellow arrows). (D–F) Electron microscopy shows that the lipid is contained within variably sized vacuoles, some of which are large enough to displace the normal RPE melanin content. BRU, Bruch's membrane; IS, inner segment; ONL, outer nuclear layer; OS, outer segment; RPE, retinal pigment epithelium.