Table 1.
List of antibodies used in this study
| Antibody | Host | Immunoblot | Immunostaining | Supplier |
|---|---|---|---|---|
| LC3 | rabbit | 1:1000 | 1:200 | Novus Biologicals, 4108 |
| ATG5 | rabbit | 1:1000 | — | Novus Biologicals, NB100–53818S |
| ATG12 | rabbit | 1:1000 | — | Cell Signaling Technology, 4180 |
| SQSTM1/p62 | rabbit | 1:1000 | — | Novus Biologicals, NBP1–48320S |
| C3 | mouse | 1:500 | 1:100 | Santa Cruz Biotechnology, cs-28294 |
| ITGB1/CD29 | mouse | 1:100 | Biolegend, 303001 | |
| CD68 | rat | — | 1:200 | AbD Serotec, MCA1957T |
| CTSD/cathepsin D | goat | 1:200 | — | Santa Cruz Biotechnology, sc-6487 |
| Cre-recombinase | mouse | 1:1000 | 1:500 | Millipore, MAB3120 |
| Cre-recombinase | rabbit | — | 1:1000 | Novagen, 69050–3 |
| RB1CC1/FIP200 | rabbit | 1:1000 | — | Proteintech, 10041–2-AP |
| Isolectin IB4 488 Conjugate | Griffoniasimplicifolia | — | 1:50 | Invitrogen, 121411 |
| IBA1 | goat | — | 1:500 | Novus Biologicals, NB100–1028 |
| PTK2/FAK | rabbit | 1:1000 | — | Cell Signaling Technology, 3285 |
| Phospho-PTK2 | rabbit | 1:500 | — | Cell Signaling Technology, 3284 |
| MAPK8/JNK1-MAPK9/JNK2 | rabbit | 1:1000 | — | Cell Signaling Technology, 9258 |
| Phospho MAPK8/JNK1-MAPK9/JNK/2 | rabbit | 1:500 | — | Cell Signaling Technology, 9234 |
| LAMP1 | mouse | 1:2000 | — | EnCor Biotechnology, MAC-6E2 |
| Nitrotyrosine | mouse | — | 1:100 | Santa Cruz Biotechnology, sc-32731 |
| RPE65 | mouse | — | 1:200 | Courtesy of Dr. Thompson 58 |
| TOMM40 | rabbit | — | 1:200 | Santa Cruz Biotechnology, sc-11414 |
| Ubiquitin | rabbit | — | 1: 600 | Cell Signaling Technology, 3933 |
| TJP1/ZO-1 | rabbit | — | 1:100 | Invitrogen, 61–7300 |
| GAPDH | mouse | 1:60,000 | — | Ambion Applied Biosystems, AM4300 |
Ratios listed are the dilution used for each particular experiment.