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TPT-induced autophagy in A549 and HeLa cells. (A) A549 and HeLa cells were transiently transfected with YFP-LC3 plasmids. Cells were treated with or without 3 μg/mL TPT for 24 h. Green YFP autofluorescence (YFP-LC3) was analyzed by confocal microscopy (100×). (B) A549 and HeLa cells were treated with various concentrations of TPT for 24 h or with 3 μg/mL TPT for the indicated periods. The expression levels of MAP1LC3/LC3 and SQSTM1/p62 protein were analyzed by immunoblotting.
