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. 2014 Oct 30;10(11):2036–2052. doi: 10.4161/auto.34398

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Monoallelic Becn1 loss results in KRT6 upregulation in mammary epithelial cells and tissues. (A) Microarray analysis was performed on samples (three each) of Becn1^+/+ and Becn1^+/− apoptosis-competent and apoptosis-defective iMMECS, as well as mammary tumors derived from orthotopic implantation of apoptosis-defective iMMECs in nude mice. KRT6 expression relative to that in apoptosis-competent Becn1^+/+ iMMECs is presented as means ± SDs (n = 3). (B–D) Becn1^+/+ (left) and Becn1^+/− (right) with DAPI (blue) by IF: (B) More KRT6-expressing cells are seen in Becn1^+/− apoptosis-competent iMMECs (top) and Becn1^+/− apoptosis-defective iMMECs (bottom) under normal growth conditions. (C) More KRT6-expressing (green, top) and KRT14-expressing (red, bottom) cells are seen in allograft mammary tumors generated by Becn1^+/− apoptosis-defective iMMECs. (D) MGs from 5-wk-old (top) and 9-mo-old (bottom) Becn1^+/− mice reveals increased number of KRT6-positive (green) cells that are not KRT8-positive (red). (E) Costaining for KRT6 (purple), CD24 (red), CD29 (green) in MGs from 9-mo-old Becn1^+/− mice reveals that KRT6-positve cells colocalize with mammary progenitor cell markers. Scale bar: (B and D) 30 μm; (C and E) 37.5 μm.