Figure 2.

P2RX7-dependent LP formation is linked to autophagy induction in Dmd^mdx myoblasts. (A) Representative images showing P2RX7-dependent increases in Lucifer Yellow uptake (LY, green) and autophagy induction (LC3 aggregation, red) in Dmd^mdx myoblasts exposed to 1 mM BzATP. LY uptake was observed in cells displaying high levels of autophagy, where dye colocalized with LC3 immunofluorescence in distinct clusters. (B) ImageJ quantification of LC3 clusters number, size, and density in LY-positive and LY-negative cells using 3D compilations of z-stacked confocal images, with an example image shown. Mean +/- SE, n = 10, P = 0.0001***.