Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 16;14:104. doi: 10.1186/s12934-015-0271-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Samazan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Production of secreted rHtrA proteins in L. lactis. rHtrA₁ and rHtrA₂ proteins, together with a recombinant form of the staphylococcal nuclease [4] as a positive control, were produced and secreted in L. lactis. Recombinant strains [MG1363(pLB145) in lanes 1 and 2, MG1363(pVE8126) in lane 3 and MG1363 (pVE8127) in lane 4] were grown to the exponential phase in rich M17 medium supplemented with glucose and buffered with β-glycerophosphate, in flasks. EDTA at 500 μM was added to the cultures to induce (lanes 2–4) or not (lane 1, as a negative control) recombinant protein production. After further growth, culture supernatants were recovered, concentrated, precipitated and finally subjected to SDS-PAGE and Coomassie Brilliant Blue staining. Culture supernatants all show the major lactococcal secreted protein, Usp45 [9] either alone (lane 1) or together with one of the recombinant proteins (lanes 2–4). Usp45 (lanes 1–4), rHtrA₁ (lane 3), rHtrA₂ (lane 4) and for recombinant staphylococcal nuclease (lane 2), both the secreted form, NucB, and its maturation product (released by lactococcal HtrA protease), NucA [4, 10], are indicated by arrows.