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. 2015 Jul 16;14:104. doi: 10.1186/s12934-015-0271-z

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© Samazan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purification of rHtrA proteins. Each rHtrA protein (rHtrA₁ in a and rHtrA₂ in b), after production and secretion in L. lactis (by either strain MG1363(pVE8126) in a or strain MG1363(pVE8127) in b) grown in fermenter as described in Figure 3, was purified by affinity. At each purification step, an SDS-PAGE analysis followed by Coomassie Brilliant Blue staining was performed. Lane 1 concentrated supernatant from an induced culture after growth in fermenter, Lane 2 flow through, Lanes 3 and 4 washing number 1 and 2, Lanes 5–7 elution fractions number 1–3. MWM molecular weight marker.