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. 2015 Jul 16;14:104. doi: 10.1186/s12934-015-0271-z

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© Samazan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cytometry analysis of WT HtrA protein exposure at the staphylococcal cell surface. The cell surface exposure of WT HtrA₁ (A) and HtrA₂ (B) proteins from strain Lowenstein was analyzed by flow cytometry. Staphylococcal cells were grown either in TSB medium (a), TSB supplemented with 2,2′ Dipyridyl 1 mM (b), or SATA-2 medium (c) till early exponential phase, late exponential phase or late stationary phase. Shaded and white areas represent cells incubated with antibodies against SP02 adjuvant or each rHtrA protein (rHtrA₁ in A and rHtrA₂ in B) respectively.