Table 1.
Trypsin-like serine peptidases identified by mass spectrometry in the midgut of Culex quinquefasciatus females fed on sugar
| Identified Proteins | Accession Number | Molecular Weight | Mascot exclusive peptides | Mascot total spectra | Mascot Coverage % | Peptide sequence identified by MASCOT | Mascot Ion score | Peptide sequences identified by ProLuCID | ProLuCID unique peptides | ProLuCID total spectra | ProLuCID Coverage % |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Trypsin 4 OS = Culex quinquefasciatus GN = CpipJ_CPIJ017414 | B0XCW2_CULQU | 28 kDa | 3 | 13 | 18 | 6 | 29 | 28 | |||
| (R)VGSSYDYQGGTVIDVAGMTIHPR(Y) | 35.21 | (R)VGSSYDYQGGTVIDVAGMTIHPR(Y) | |||||||||
| (K)DFDFALLR(L) | 52.94 | (K)DFDFALLR(L) | |||||||||
| (K)GCAQPDYYGVYADVEK(A) | 39.12 | (K)GCAQPDYYGVYADVEK(A) | |||||||||
| (K)NMLCAGYDEGLR(D) | |||||||||||
| (R)LSWIGVR(V) | |||||||||||
| (R)ENYAESR(L) | |||||||||||
| Trypsin 7 OS = Culex quinquefasciatus GN = CpipJ_CPIJ017964 | B0XES8_CULQU | 27 kDa | 4 | 14 | 18 | 5 | 25 | 18 | |||
| (R)GGQLIAVTR(K) | 53.31 | (R)GGQLIAVTR(K) | |||||||||
| (R)DYALLNLAK(S) | 50.34 | (R)DYALLNLAK(S) | |||||||||
| (R)AVDVPIADHDR(C) | 24.69 | (R)AVDVPIADHDR(C) | |||||||||
| (K)DACLGDSGGPLTCSGK(V) | 49.46 | (K)DACLGDSGGPLTCSGK(V) | |||||||||
| (F)M*LCAGYDAGGK(D) | |||||||||||
| Trypsin-5 OS = Culex quinquefasciatus GN = CpipJ_CPIJ015103 | B0X667_CULQU | 30 kDa | 3 | 7 | 16 | 3 | 14 | 16 | |||
| (K)IIGGFPAEQGDTLHQVSIR(F) | 35.64 | (K)IIGGFPAEQGDTLHQVSIR(F) | |||||||||
| (K)GCGLAAYPGIYSDVAYYR(G) | 29.61 | (K)GCGLAAYPGIYSDVAYYR(G) | |||||||||
| (R)GWIDSCLAGK(C) | 31.7 | (R)GWIDSCLAGK(C) | |||||||||
| Trypsin-1 OS = Culex quinquefasciatus GN = CpipJ_CPIJ007079 | B0WIS4_CULQU | 29 kDa | 3 | 6 | 18 | 6 | 10 | 34 | |||
| (R)IVGGFEISIADAPHQVSLQSR(G) | 51.51 | (R)IVGGFEISIADAPHQVSLQSR(G) | |||||||||
| (K)HASGGSVISIK(R) | 26.21 | (K)HASGGSVISIK(R) | |||||||||
| (R)AAYVPAYNQNQCNSAYAR(Y) | 29.15 | (R)AAYVPAYNQNQCNSAYAR(Y) | |||||||||
| (K)DACQGDSGGPLVADGK(L) | 33.42 | (R)NTIDYDYSLLELK(S) | |||||||||
| (R)GSHICGGSIISPK(W) | |||||||||||
| (K)WILTAAHCTDGASVSNLR(I) | |||||||||||
| Trypsin 2 OS = Culex quinquefasciatus GN = CpipJ_CPIJ005273 | B0WE94_CULQU | 28 kDa | 2 | 2 | 12 | 5 | 13 | 30 | |||
| (R)LEFGHAVQPVDLVR(D) | 19.14 | (R)LEFGHAVQPVDLVR(D) | |||||||||
| (R)DEPADESQSLVSGWGDTR(S) | 27.7 | (R)DEPADESQSLVSGWGDTR(S) | |||||||||
| (R)WVLTAAHCTENTDAGIYSVR(V) | |||||||||||
| (R)GVLVPLVNR(E) | |||||||||||
| (K)LGMPVTESMICAGFAK(E) | |||||||||||
| Serine protease1/2 OS = Culex quinquefasciatus GN = CpipJ_CPIJ003826 PE = 3 SV = 1 | B0W9S9_CULQU | 30 kDa | 2 | 3 | 18 | 2 | 3 | 10 | |||
| (R)TGETFVDNQATVSGFGR(T) | 35.91 | (R)TGETFVDNQATVSGFGR(T) | |||||||||
| (R)TVDGGPVSPTK(N) | 35.12 | (R)TVDGGPVSPTK(N) | |||||||||
| Serine protease SP24D OS = Culex quinquefasciatus GN = CpipJ_CPIJ015368 | B0X870_CULQU | 27 kDa | 1 | 1 | 10 | 1 | 3 | 10 | |||
| (K)LGESIEYDELSQPIALYEGDDLPK(D) | 34.98 | (K)LGESIEYDELSQPIALYEGDDLPK(D) | |||||||||
| Cationic trypsin OS = Culex quinquefasciatus GN = CpipJ_CPIJ011378 | B0WW44_CULQU | 26 kDa | 1 | 1 | 8 | ||||||
| (R)IVVHPQYAEGNLANDIAVIR(V) | 32.92 |