Table 3.
In silico characterization of trypsin-like serine peptidases identified in the midgut of Culex quinquefasciatus females fed on sugar
| Uniprot accession number | Protein name | Target P prediction ① | Signal P prediction ② | TMHMM prediction ③ | N-Glycosylation prediction ④ | O-Glycosylation prediction ⑤ | Exon number ⑥ | Paralogues number ⑥ | Supercontig ⑥ |
|---|---|---|---|---|---|---|---|---|---|
| B0WIS4 | Trypsin 1 | S (0.901) | 23^24 (0.761) | No | 158-NETV (0.7243) | 36-T (0.627995)/40-S (0.689668) | 1 | 36 | 3.14 |
| B0WE94 | Trypsin 2 | S (0.910) | 18^19 (0.818) | No | No | No | 2 | 36 | 3.94 |
| B0XCW2 | Trypsin 4 | S (0.973) | 22^23 (0.935) | inside: 1-6/Tmhelix:7-26/outside: 27-258 | 27-NGTQ (0.8040) | No | 2 | 36 | 3.91 |
| B0X667 | Trypsin 5 | S (0.952) | 17^18 (0.855) | inside: 291-293/Tmhelix: 268-290/outside: 1-267 | 65-NRTV (0.6702)/183-NVTV (0.8306) | 151-T (0.653105)/159-S (0.523482) | 3 | 13 | 3.59 |
| B0XES8 | Trypsin 7 | S (0.832) | 21^22 (0.665) | No | 106-NVTF (0.6360) | No | 3 | 36 | 3.11 |
| B0X870 | SP24D | S (0.891) | 20^21 (0.801) | No | 69-NGSV (0.6998)/75-NLSV (0.6183) | No | 2 | 24 | 3.66 |
| B0W9S9 | Serine protease 1/2 | S (0.960) | 26^27 (0.750) | No | No | 44-S (0.785129) | 2 | 38 | 3.54 |
| B0WW44 | Cationic trypsin | S (0.926) | 20^21 (0.794) | No | No | No | 3 | 24 | 3.33 |
① TargetP 1.1 Server. Prediction of the subcellular location of trypsin. S = secreted. The number into the parenthesis indicates the probability
② SignalP 4.0 Server. Prediction of presence and location of signal peptide cleavage sites in the trypsin sequences. The numbers indicates the number of the amino acid residues involved in the cleavage. The number into the parenthesis indicates the probability
③ TMHMM 2.0 Server. Prediction of transmembrane helices in proteins. Tmhelix: transmembrane helix
④ NetNglyc 1.0 Server. Prediction of N-Glycosylation sites based on the presence of Asn-Xaa-Ser/Thr motifs. The number into the parenthesis indicates the probability
⑤ NetOglyc 4.0 Server. Prediction of mucin type GalNAc O-glycosylation sites
⑥ According to VectorBase database