Table 4.
Proteotypic peptides from trypsins identified by MS/MS. Proteotypic peptides were predicted using SKYLINE software
| Uniprot accession number | Protein name | Peptides identified by MS/MS | Species-specific peptide | Trypsin unique peptide | Other proteins witn the same peptide |
|---|---|---|---|---|---|
| B0WIS4 | Trypsin 1 | R.AAYVPAYNQNQCNSAYAR.Y | Yes | No | Q1KWX6 (Trypsin-like fragment) - C. quinquefasciatus |
| R.IVGGFEISIADAPHQVSLQSR.G | Yes | Yes | - | ||
| R.GSHICGGSIISPK.W | Yes | Yes | - | ||
| R.NTIDYDYSLLELK.S | Yes | Yes | - | ||
| K.WILTAAHCTDGASVSNLR.I | Yes | Yes | - | ||
| K.HASGGSVISIK.R | - | - | Not predicted by Skyline | ||
| B0WE94 | Trypsin 2 | R.DEPADESQSLVSGWGDTR.S | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens |
| R.LEFGHAVQPVDLVR.D | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens | ||
| R.GVLVPLVNR.E | No | No | Q962G7 / Q56GM3 (Trypsin) - Culex pipiens | ||
| B0XCW2 | Trypsin 4 | K.GCAQPDYYGVYADVEK.A | Yes | Yes | - |
| K.DFDFALLR.L | Yes | Yes | - | ||
| R.VGSSYDYQGGTVIDVAGMTIHPR.Y | - | - | Not predicted by Skyline | ||
| B0X667 | Trypsin 5 | K.IIGGFPAEQGDTLHQVSIR.F | Yes | Yes | - |
| K.GCGLAAYPGIYSDVAYYR.G | Yes | Yes | - | ||
| R.GWIDSCLAGK.C | Yes | Yes | - | ||
| B0XES8 | Trypsin 7 | K.DACLGDSGGPLTCSGK.V | Yes | Yes | - |
| R.DYALLNLAK.S | Yes | Yes | - | ||
| R.AVDVPIADHDR.C | Yes | Yes | - | ||
| R.GGQLIAVTR.K | - | - | Not predicted by Skyline | ||
| B0X870 | SP24D | K.LGESIEYDELSQPIALYEGDDLPK.D | - | - | Not predicted by Skyline |
| B0W9S9 | Serine protease 1/2 | R.TGETFVDNQATVSGFGR.T | Yes | No | C. quinquefasciatus - Q23731 (Serine protease) |
| R.TVDGGPVSPTK.N | Yes | No | C. quinquefasciatus - Q23731 (Serine protease) | ||
| B0WW44 | Cationic trypsin | R.IVVHPQYAEGNLANDIAVIR.V | Yes | Yes | - |